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64 BD FACSAria II User’s Guide

Typically, when setting up for sorting, you use the Amplitude to set the required

drop breakoff, and copy the generated Drop 1 value to the target field. Then, you

turn on the Sweet Spot to maintain the drop breakoff. When the Sweet Spot

detects a >1-pixel difference between the target Gap setting and the actual Gap, it

adjusts the Amplitude to reduce the Gap. When a >2-pixel difference is detected,

the Sweet Spot stops sorting temporarily (stops charging the stream) until the

Gap is restored. Note that when the sort is paused, the sample continues to flow.

Once the Gap is back within range, sorting automatically resumes.

For information on setting the breakoff, see Setting Up the Breakoff on page 119.

Frequency field

(continued)

In general, the drop drive frequency should not need adjustment. We

recommend using the default values that are entered with each Sort

Setup mode.

Drop 1 field The distance between the top of the image and the center of the first

broken-off drop, from 100–600 pixels.

When you enter a value and turn on the Sweet Spot, the cytometer

automatically adjusts the amplitude to attain your target value.

Note that not all Drop 1 targets are attainable. The Drop 1 value

jumps in whole increments of approximately 57 pixels according to

your drop spacing.

The same Drop 1 setting should be used from day to day. A difference

of up to 10 pixels between the target value and the actual value is

acceptable.

Gap field The gap between the stream breakoff and the top of the first drop.

The default Gap setting for a 70 micron nozzle is 6 pixels.

Table 2-1 Breakoff window controls (continued)

Control Description

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Aria 8.0 Guida Utente Pagina 64