Aria 8.0 Guida Utente Pagina 208

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208 BD FACSAria II User’s Guide
Insert the sample line into the sample injection chamber fitting. Push the
tubing from the top until it is slightly above the bottom of the chamber
viewing window. Finger-tighten the nut on top of the chamber so the
sample line is secure.
8 Check the fitting connections at both ends to make sure they are not
leaking.
Turn on the stream, load a tube of DI water, and make sure none of the
fittings are leaking. If needed, unload the tube, turn off the stream, and
tighten the fittings. After tightening, if leaking still occurs, replace the
ferrule.
9 Verify the length of the sample line.
The sample line should not bow or bend when a tube is loaded. If you need
to adjust the length, unscrew the nut on top of the sample injection
chamber, adjust the length, and tighten the nut again.
Changing the Secondary Sample Line
This section describes changing the secondary sample line (see Figure 6-9 on
page 204). There is a different fitting at each end of the tubing, so the procedure
is divided into two sections.
Pinch Valve End
The procedure to replace the secondary sample line at the pinch valve end is the
same as for the primary sample line, except the replacement line is a 7-inch
length. Refer to Assembling the Collet Fitting at the Pinch Valve on page 205.
Do not overtighten the nut and do not use tools. Over-tightening the nut
can kink or damage the tubing.
Make sure all fittings are securely tightened. If any fitting is loose, the
tubing could detach during high-pressure operation, exposing the
operator to potentially biohazardous sample spray.
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