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101

Chapter 2: Theory of Operation 45

a constant pressure. You can view the current sheath pressure setting using the

Cytometer > Sheath Pressure command (Figure 2-2).

Figure 2-2 Sheath pressure level

After fluidics startup, sheath flow is controlled using the Stream button in the

Breakoff window (Figure 2-3). When clicked, the button changes from a red “X”

to a green checkmark, and sheath flows through the cuvette flow cell at the rate

that is specified in the Sheath Pressure window. (See Drop Formation on page 61

for a complete description of the Breakoff window.)

Figure 2-3 Stream control in Breakoff window

As a general rule, the sheath pressure level is set by choosing a Sort Setup mode

from the Sort menu, rather than by adjusting the Sheath Pressure control. Each

Sort Setup mode is optimized at a preset sheath pressure. If you change the sheath

pressure, a multitude of other values will be affected and need updating,

including the drop drive frequency, drop charge levels, laser delay, area scaling

factor, and other values. For more information, see Sort Setup on page 101.

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