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230 BD FACSAria II User’s Guide

Using Custom Optical Filters

If you want to install a custom filter or dichroic, the filter should comply with the

following specifications.

For the longpass filters, the surface that faces the center of the octagon/trigon

should be coated directly on its surface, not between two or more pieces of glass.

The coating should transmit !70% of the wavelength range you want the filter to

transmit, with a minimum transmission of !50%, and it should reflect >90% of

the wavelength range you want the filter to reflect.

The opposite surface (facing away from the center) should be coated with an

anti-reflective coating with a minimum reflection of d1% of the wavelength

range you want the filter to reflect.

Note that filters must be installed in front of each PMT to block unwanted laser

light. For the dichroic, carefully assemble the glass filter in the holder using the

retaining spring. Assemble the filter with the arrow pointing toward the center of

the octagon or trigon. The angle of the dichroic is critical to achieving optimal

results.

Note that any time you modify a detector array, you will need to create a new

cytometer configuration to identify which PMT (identified by laser color and

letter) will detect the emitted light. See Custom Configurations on page 90 for

instructions.

Table 6-4 BD FACSAria II filter specifications

Filter Characteristic Dichroic LP Filters BP Filters

Diameter 0.622 ±0.003 in. 1.00 ±0.010 in.

Thickness 0.125 ±0.005 in. 0.12–0.35 in.

Minimum clear aperture 0.562 in. 0.85 in.

Incident angle 11 ±1° 0°

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